Robyn Erland shared a link (from Facebook).
They were doing this in the 80's: J Gen Virol. 1989 Aug;70 ( Pt 8):1987-93.
Bovine leukaemia virus packaging cell line for retrovirus-mediated gene transfer.
Ban J1, First NL, Temin HM.
Author information
Abstract
Retroviral packaging cell lines were constructed by using the gag-pol gene of spleen necrosis virus, the gag-pol gene of Moloney murine leukaemia virus and the env gene of bovine leukaemia virus. The plasmids containing the gag-pol genes and the plasmid containing the env gene were cotransfected into NIH/3T3 and D17 cells. The cells containing the helper virus constructs were tested for their ability to package replication-defective murine leukaemia and avian reticuloendotheliosis retrovirus vectors. The titre of vector virus produced by each of the retroviral packaging cell lines was about 10(2) colony-forming units per ml of medium. Tests for events that might result in intact replication-competent retroviruses showed no evidence for the generation of such viruses. The vector viruses were able to infect dog and rat cells. Bovine cells were infected only after their cocultivation with the retroviral packaging cell lines producing murine leukaemia virus vectors, perhaps as a result of a low concentration of receptors. http://www.ncbi.nlm.nih.gov/pubmed/2549178
Sep 24, 2015, 4:30 PM
Robyn Erland shared a link. (from Facebook).
Check this out the company Healios who makes Bovine Xenografts for the spine. That must be big business! I need some work done but I don't need another retrovirus, one is plenty!. Mouse or cow retrovirus anyone??
Oh and this was fast tracked approved by the HHS and FDA!!! Here's what they said: "This letter will allow you to begin marketing your device as described in your Section 510(k) premarket notification. The FDA finding of substantial equivalence of your device to a legally marketed predicate device results in a classification for your device and thus, permits your device to proceed to the market." Notice the word graft (as in xenograft): HEALOS®Fx Graft Mixing and Delivery
System http://www.accessdata.fda.gov/cdrh_docs/pdf8/K081758.pdf
Sep 24, 2015, 12:33 PM
Robyn Erland shared a link. (from Facebook).
Oh look one of those companies Bio-Oss(Geistlich Biomaterial),
that makes Bovine (cow) xenografts can put the product right into peoples mouth:
https://books.google.com/books?id=_4002SyMTgUC&pg=PA151&lpg=PA151&dq=bovine-derived+xenograft+definition&source=bl&ots=Y7WuDBzvI-&sig=dGWu_eA_UCXLJ-DLPGUN6F2D6Kw&hl=en&sa=X&ved=0CDkQ6AEwA2oVChMI0JvB4bCQyAIVRy6ICh2lDQpT#v=onepage&q=bovine-derived%20xenograft%20definition&f=false
Sep 24, 2015, 12:21 PM
Robyn Erland updated her status. (from Facebook)
So we all know what the Bovine retrovirus is: A deltaretrovirus is a genus of the retroviridae family. It consists of exogenous horizontally transmitted viruses found in several groups of mammals. Examples are the bovine leukemia virus and the Human T-lymphotropic virus.
Bovine leukemia virus (BLV) is a retrovirus closely related to the human T-lymphotropic virus type 1 HTLV-I.
Sep 24, 2015, 9:24 AM
Robyn Erland shared a link. (from Facebook).
Bovine leukemia virus infects dairy and beef cattle’s blood cells and mammary tissue. The RETROVIRUS is easily transmitted among cattle primarily through infected blood and milk, but it only causes disease in fewer than 5 percent of infected animals. http://news.berkeley.edu/2015/09/15/bovine-leukemia-virus-breast-cancer/
Sep 24, 2015, 9:18 AM
Check this out. If cows can spread Bovine Leukemia retrovirus via these methods how about people? BLV is a retrovirus that infects dairy and beef cattle’s lymphoid tissue, causing malignant lymphoma and lymphosarcoma in 1 to 5 percent of infected animals. The virus is transmitted to cattle primarily by direct exposure with infected blood, saliva, semen, and milk.
SALIVA??? That's not good!
Also look at this: As part of the 2007 dairy study, bulk tank milk was
collected from 534 operations with 30 or more dairy cows and tested with an Enzyme Linked-Immunosorbent Assay (ELISA) for the presence of antibodies against BLV. Results showed that 83.9 percent of U.S. dairy operations were positive for BLV
83.9 Percent INFECTION RATE??? Also not good. Here's the 2007 Bovine Leukosis Virus (BLV) on U.S. Dairy Operations, 2007 Info sheet. Oh and one thing to note is they started doing these studies when they realized the high prevalence of the retrovirus in 1996. This was their suggestion:
"The high individual animal prevalence of BLV reported in the Dairy 1996 study suggests that testing and culling seropositive animals may not be a cost
effective method to control the disease."
So it was not cost effective to either test for the retrovirus so they could remove positive animals from the food and milk supply: https://www.aphis.usda.gov/animal_health/nahms/dairy/downloads/dairy07/Dairy07_is_BLV.pdf
Sep 27, 2015, 9:58 PM
Robyn Erland shared a link. (from Facebook).
They were doing this in the 80's: J Gen Virol. 1989 Aug;70 ( Pt 8):1987-93.
Bovine leukaemia virus packaging cell line for retrovirus-mediated gene transfer.
Ban J1, First NL, Temin HM.
Author information
Abstract
Retroviral packaging cell lines were constructed by using the gag-pol gene of spleen necrosis virus, the gag-pol gene of Moloney murine leukaemia virus and the env gene of bovine leukaemia virus. The plasmids containing the gag-pol genes and the plasmid containing the env gene were cotransfected into NIH/3T3 and D17 cells. The cells containing the helper virus constructs were tested for their ability to package replication-defective murine leukaemia and avian reticuloendotheliosis retrovirus vectors. The titre of vector virus produced by each of the retroviral packaging cell lines was about 10(2) colony-forming units per ml of medium. Tests for events that might result in intact replication-competent retroviruses showed no evidence for the generation of such viruses. The vector viruses were able to infect dog and rat cells. Bovine cells were infected only after their cocultivation with the retroviral packaging cell lines producing murine leukaemia virus vectors, perhaps as a result of a low concentration of receptors. http://www.ncbi.nlm.nih.gov/pubmed/2549178
Sep 24, 2015, 4:30 PM
Robyn Erland shared a link. (from Facebook).
Check this out the company Healios who makes Bovine Xenografts for the spine. That must be big business! I need some work done but I don't need another retrovirus, one is plenty!. Mouse or cow retrovirus anyone??
Oh and this was fast tracked approved by the HHS and FDA!!! Here's what they said: "This letter will allow you to begin marketing your device as described in your Section 510(k) premarket notification. The FDA finding of substantial equivalence of your device to a legally marketed predicate device results in a classification for your device and thus, permits your device to proceed to the market." Notice the word graft (as in xenograft): HEALOS®Fx Graft Mixing and Delivery
System http://www.accessdata.fda.gov/cdrh_docs/pdf8/K081758.pdf
Sep 24, 2015, 12:33 PM
Robyn Erland updated her status. (from Facebook)
So we all know what the Bovine retrovirus is: A deltaretrovirus is a genus of the retroviridae family. It consists of exogenous horizontally transmitted viruses found in several groups of mammals. Examples are the bovine leukemia virus and the Human T-lymphotropic virus.
Bovine leukemia virus (BLV) is a retrovirus closely related to the human T-lymphotropic virus type 1 HTLV-I.
Sep 24, 2015, 10:27 AM
Robyn Erland posted in ME Clinics Best Medical Practices, Recoveries, Research. (from Facebook)
Bovine leukemia virus infects dairy and beef cattle’s blood cells and mammary tissue. The RETROVIRUS is easily transmitted among cattle primarily through infected blood and milk, but it only causes disease in fewer than 5 percent of infected animals. http://news.berkeley.edu/2015/09/15/bovine-leukemia-virus-breast-cancer/
Sep 24, 2015, 9:24 AM
Much more on this to come...
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